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nta data and ihc expression score analysis  (GraphPad Software Inc)
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Enrichment and characterization of cell line and tissue explant EVs. a) Surgical resections of healthy FT or tumor tissues were minced and used to initiate short-term tissue explants (cultured for 24 h) followed by collection of the conditioned media and processed by differential ultracentrifugation to enrich for EVs. Likewise, conditioned media from the FT and ovarian cancer cell lines shown was collected and processed. Created with BioRender.com b) Representative <t>NTA</t> <t>data.</t> c) Sixty (60) EV particles were imaged, and their size was measured for representative samples by TEM at x30K magnification. d) Shown are representative SP-IRIS and fluorescence (ExoView) data of FT tissue explant derived EVs displaying the common tetraspanins observed with cell-line derived EVs: CD9 (blue), CD63 (pink) and CD81 (green).
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Enrichment and characterization of cell line and tissue explant EVs. a) Surgical resections of healthy FT or tumor tissues were minced and used to initiate short-term tissue explants (cultured for 24 h) followed by collection of the conditioned media and processed by differential ultracentrifugation to enrich for EVs. Likewise, conditioned media from the FT and ovarian cancer cell lines shown was collected and processed. Created with BioRender.com b) Representative NTA data. c) Sixty (60) EV particles were imaged, and their size was measured for representative samples by TEM at x30K magnification. d) Shown are representative SP-IRIS and fluorescence (ExoView) data of FT tissue explant derived EVs displaying the common tetraspanins observed with cell-line derived EVs: CD9 (blue), CD63 (pink) and CD81 (green).

Journal: Research Square

Article Title: Lineage specific extracellular vesicle-associated protein biomarkers for the early detection of high grade serous ovarian cancer

doi: 10.21203/rs.3.rs-2814022/v1

Figure Lengend Snippet: Enrichment and characterization of cell line and tissue explant EVs. a) Surgical resections of healthy FT or tumor tissues were minced and used to initiate short-term tissue explants (cultured for 24 h) followed by collection of the conditioned media and processed by differential ultracentrifugation to enrich for EVs. Likewise, conditioned media from the FT and ovarian cancer cell lines shown was collected and processed. Created with BioRender.com b) Representative NTA data. c) Sixty (60) EV particles were imaged, and their size was measured for representative samples by TEM at x30K magnification. d) Shown are representative SP-IRIS and fluorescence (ExoView) data of FT tissue explant derived EVs displaying the common tetraspanins observed with cell-line derived EVs: CD9 (blue), CD63 (pink) and CD81 (green).

Article Snippet: For NTA data and IHC expression score analysis, GraphPad Prism version 8 was used for performing unpaired Mann-Whitney t-tests and calculating p-values to compare between the samples.

Techniques: Cell Culture, Fluorescence, Derivative Assay